Co-culture of ginsenosides Rg1 and neural stem cells：promoting proliferation role and protective effect
10.3969/j.issn.2095-4344.2015.10.019
- VernacularTitle:人参皂苷Rg1与神经干细胞共培养的促增殖和保护作用
- Author:
Jianzhong SUN
;
Xinwei LIU
;
Huapeng GUAN
;
Peng ZHANG
;
Qi LIU
;
Jun YANG
;
Qunfeng GUO
;
Bin NI
- Publication Type:Journal Article
- Keywords:
Ginsenosides;
Spinal Cord Injuries;
Neural Stem Cels;
Cel Proliferation;
Brain-Derived Neurotrophic Factor;
Transforming Growth Factor beta
- From:
Chinese Journal of Tissue Engineering Research
2015;(10):1580-1584
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Chinese herb extracts can restore and protect the nervous system of rats through intervention of neural stem cels. OBJECTIVE:To explore the role of ginsenosides Rg1 in the proliferation and protection of neural stem cels. METHOD:Sprague-Dawley rats at pregnant 19 days were dissected to take out fetal rats, and then the hippocampal tissues from fetal rats were isolated to extract neural stem cels. Neural stem cels were co-cultured with DMEM/F12 medium containing 50 g/L ginsenosides Rg1 as intervention group, with DMEM/F12 medium as blank control group, and with DMEM/F12 containing 0.64% phenol as positive control group, respectively. MTT assay was used to detect the proliferation of neural stem cels in each group, and western blot method to detect the protein expression of brain-derived neurotrophic factor and transforming growth factor-β in neural stem cels. RESULTS AND CONCLUSION:Rat neural stem cels were round single cels with clear border at early period after isolation but at 2 days after inoculation, the cels were adherent and aggregated into smal cel spheres. Compared with the blank control group, the proliferative rate of neural stem cels was significantly increased in the ginsenosides Rg1 group (P < 0.05), but decreased in the positive control group (P < 0.05). Compared with the blank control group, in the ginsenosides Rg1 group, the expression of brain-derived neurotrophic factor was elevated, and the expression of transforming growth factor-β was reduced, indicating ginsenosides Rg1 has a certain effect to promote the proliferation of neural stem cels as wel as to protect the neural stem cels.
