Homing time window of allogeneic bone marrow stem cells transplanted into Chinese miniature pigs with myocardial infarction
10.3969/j.issn.2095-4344.2015.10.014
- VernacularTitle:中华小型猪心肌梗死后移植同种异体骨髓间充质干细胞归巢时间窗
- Author:
Liping XIANG
;
Lina GUAN
;
Qi SHI
;
Yuming MU
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cel Transplantation;
Myocardial Infarction
- From:
Chinese Journal of Tissue Engineering Research
2015;(10):1551-1555
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Due to the different transplantation time after myocardial infarction, the homing ability of bone marrow mesenchymal stem cels to damaged tissues as wel as the repairing role wil be very different. OBJECTIVE:To explore the optimal window time for the homing of bone marrow mesenchymal stem cels to the myocardial tissue after myocardial infarction. METHODS: Eighteen Chinese miniature pigs were modeled by the ligation of left anterior descending coronary artery. BrdU-labeled bone marrow mesenchymal stem cels were injectedvia the coronary artery at 1 day, 3 days, 1 week, 2 weeks, 3 weeks, 4 weeks after modeling, respectively. Then, the animals were kiled at 3 days after cel transplantation to detect the home of bone marrow mesenchymal stem cels in the infarct zone. RESULTS AND CONCLUSION:BrdU-labeled positive cels with brown nuclei were visible at 1 day, 3 days, 1 week, 2 weeks, 3 weeks, and 4 weeks after myocardial infarction, especialy at 1 week after myocardial infarction (P < 0.05). It indicates that the best homing window for bone marrow mesenchymal stem cels was at week after myocardial infarction, when the stem cel transplantation is given to be able to promote myocardial repair.
