Effect of salidroside on radiation-induced bone marrow adipogenesis
10.3760/cma.j.issn.0254-5098.2014.10.004
- VernacularTitle:红景天苷对急性辐射损伤小鼠骨髓脂肪细胞生成的影响
- Author:
Jincan ZHU
;
Xiaoyu CHEN
;
Chengcheng LIU
;
Aizhen ZHU
;
Shantao LIU
;
Gexiu LIU
- Publication Type:Journal Article
- Keywords:
Radiation;
Adipocyte;
Bone marrow microenvironment;
Salidroside
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(10):735-738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential and underlying molecular mechanism of salidroside in ameliorating radiation-induced bone marrow adipogenesis and stimulating hematopoiesis.Methods The female BALB/c mice aged 6-7 weeks were randomly divided into normal control group,radiation group and salidroside group.The radiation group and salidroside group were irradiated with 6.0 Gy of 60Co γ-rays.The salidroside group was intraperitoneally injected with 30 mg· kg-1 · d-1 salidroside at 12 h and then every day until 8th d after radiation.The normal control group and radiation group were treated with equal volume of saline as control of salidroside.At 14 d after radiation,the mice weight,peripheral blood count,femur bone marrow histology,and the proportion of adipocyte area were measured,and the expressions of PPAR-γ and FABP4 were detected by q-PCR.Results After irradiation,the numbers of white blood cells,hemoglobin and platelet in peripheral blood were reduced obviously,and the percentage of adipocyte area was increased significantly.Compared with mice in the radiation group,salidroside inhibited adipogenesis and reduced the proportion of adipocyte area (t =13.31,P ＜ 0.05) by reducing the expressions of PPAR-γ and FABP4 (t =8.64,13.19,P ＜ 0.05).The number of white blood cells was partly recovered at 7 d after irradiation (t =5.80,P ＜ 0.05).Both white blood cells and hemoglobinin in peripheral blood of the salidroside group were higher than those in the radiation group at 14 d after irradiation.Conclusions Salidroside could inhibit radiation-induced bone marrow adipogenesis and regulate bone marrow microenvironment,thereby promotes hematopoietic recovery in mice after radiation injury.
