Study on heat shock protein 90α and β in peripheral blood mononuclear cells in mayasthenia gravis children treated with glucocorticoid
10.3760/cma.j.issn.1008-1372.2015.02.024
- VernacularTitle:糖皮质激素治疗重症肌无力患儿与外周血单个核细胞中热休克蛋白90α、β的关系
- Author:
Running LIU
;
Zhi HUANG
- Publication Type:Journal Article
- Keywords:
Glucocorticoids/TU;
Myasthenia gravis/BL/DT;
HSP90 heat-shock proteins/BL;
Monocytes
- From:
Journal of Chinese Physician
2015;17(2):243-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of heat shock protein(HSP)90α and HSP90β in peripheral blood mononuclear cells (PBMC) in myasthenia gravis (MG) children before and after glucocorticoid (GC) treatment,and normal control groups.Methods Fresh bloods were collected from 20 cases of children with MG before and after treatment and 19 healthy children.PBMCs were isolated,followed by measurement of the expression levels of HSP90α and β by immunocytochemistry,and HSP90α and β mRNAs by reverse transcription and polymerase chain reaction (RT-PCR).Results The positive cell ratio of HSP90α andβ of MG children before and after of GC treatment was significantly higher than control groups (P ＜ 0.05),and their mRNA expression levels was higher than control groups (P ＜ 0.05).HSP90β in MG children after GC treatment was mainly expressed in the nucleus(P ＜ 0.05).Conclusions HSP90α and β in PBMC of MG children before and after GC treatment were significantly higher than those of normal control groups.HSP90β in MG Children after GC treatment was significantly increased.The present results suggest HSP90β promote GC receptor binding and enhance GC transportation,which promotes and strengthens the physiological and pharmacological effects of GC to improve the clinical symptom of MG children.
