Expression and clinical significance of Th17 and Treg cells in peripheral blood of patients with ovarian cancer
10.3969/j.issn.1000-484X.2015.04.022
- VernacularTitle:卵巢癌患者外周血Th17／Treg细胞的检测及其临床意义
- Author:
Sufang SHAO
;
Chunmei YU
- Publication Type:Journal Article
- Keywords:
Th17 cell;
Regulatory T cell;
Ovarian cancer;
Interleukin-17
- From:
Chinese Journal of Immunology
2015;(4):537-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the levels of T help cell 17 (Th17)/regulatory T cell (Treg) in the peripheral blood of patients with ovarian cancer and explore their clinical significances.Methods:55 patients with ovarian cancer and 60 normal controls were enrolled.The percentage of Th17/Treg cells were detected by flow cytometry (FCM).The expression of retinoid-related orphan receptor gamma-t ( RORγt)/foxhead winged-helix box protein 3 ( Foxp3) mRNA was measured by real time PCR and the plasma levels of IL-17/TGF-β1 were detected by enzyme linked immunosorbent assay (ELISA).Results: The percentage of CD4+IL-17+/CD4+T cells,the mRNA expression of RORγt and the plasma levels of IL-17 increased significantly in patients with ovarian cancer than in normal controls (P<0.05).Compared with normal controls,the percentage of CD4+Foxp3+Treg/CD4+T cells,the mRNA expression of Foxp3 and the plasma levels of TGF-β1 increased significantly in patients with ovarian cancer ( P<0.05).Conclusion:The imbalance of Th17/Treg cells may exist in the peripheral blood of patients with ovarian cancer.Th17/Treg cells may participate the pathology of ovarian cancer.
