Determination of Clindamycin Phosphate in Compound Sodium Phenytoin Gels by HPLC
- VernacularTitle:HPLC法测定复方苯妥英钠凝胶剂中克林霉素磷酸酯的含量
- Author:
Ji ZEWU
;
Zhang FANG
;
Chen PING
- Publication Type:Journal Article
- Keywords:
Clindamycin phosphate;
Compound phenytoin sodium gels;
HPLC
- From:
China Pharmacist
2015;(4):690-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of clindamycin phosphate in compound phenytoin sodi-um ge1s. Methods:The HPLC analysis was carried out on a ZORBAX SB-C18 column(250 × 46 mm,5 μm)with 0. 1 mol·L-1 KH2PO4 solution(adjusting pH to 2. 5 with H3PO4 solution)-acetonitrile(75:25)as the mobile phase at the flow rate of 0. 8 ml· min-1 . The detection wavelength was 210 nm,the column temperature was 25℃ and the injection volume was 10μl. Results:The lin-ear range of clindamycin phosphate was 3. 00-18. 00 μg(r=0. 999 5). The average recovery was 101. 11%(RSD=0. 34%,n=6). Conclusion:The method is simple,sensitive and reproducible,and can be used in the determination of clindamycin phosphate in com-pound phenytoin sodium gels.
