Effect of energized fusion protein anti-CD2o (Fab)-LDM on proliferation and DNA damage of human lymphoma cell line BJAB
10.3760/cma.j.issn.1009-9921.2013.08.005
- VernacularTitle:强化融合蛋白抗CD20(Fab)-力达霉素对淋巴瘤BJAB细胞增殖和DNA损伤的作用
- Author:
Xiang YUAN
;
Linlin JIANG
;
Shannan CAO
;
Xiaoyun ZHANG
;
Huaifeng QI
;
Dongsheng XIONG
;
Xiaolong LIAO
- Publication Type:Journal Article
- Keywords:
Energized fusion protein;
Anti-CD20 antibody;
BJAB cells;
Cell cycle arrest
- From:
Journal of Leukemia & Lymphoma
2013;22(8):462-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth inhibition and DNA damage of energized fusion protein anti-CD20(Fab)-LDM on B JAB cells in vitro.Methods The binding activity of fusion protein anti-CD20 (Fab)-LDP to B JAB cells was studied by flow cytometry and confocal laser scanning microscopy.MTT assay was used to study the energized fusion protein anti-CD20(Fab)-LDM on cell growth of B JAB cells.Comet assay was employed to detect DNA damage in B JAB cells.The cell growth cycle of BJAB was analyzed by FACS.Results The recombinant fusion protein anti-CD20 (Fab)-LDP possessed an significant target affinity towarded BJAB cells.The energized fusion protein anti-CD20(Fab)-LDM showed obvious inhibition on proliferation,as well as induced potent DNA damage in B JAB cells in vitro compared with lidamycin.B JAB cells treated with energized fusion protein anti-CD20 (Fab)-LDM showed S phase cell cycle.Conclusion The energized fusion protein anti-CD20 (Fab)-LDM could target binding to BJAB cells and significantly inhibit the proliferation of B JAB cells by inducing DNA damage and S phase arrest.
