Growth inhibition and apoptosis induction to imatinib-resistant cell line K562/G01 by Bortezomib

VernacularTitle:硼替佐米对伊马替尼耐药细胞株K562/G01增殖抑制和诱导凋亡作用的研究
Author: Ying ZHOU ; Lianigming MA ; Yanyan NIU ; Ruirui REN
Publication Type:Journal Article
Keywords: Leukemia,myeloid,chronic; Neoplasms,experimental; Bortezomib; Imatinib; K562/G01 cell; Drug resistance,neoplasms
From: Journal of Leukemia & Lymphoma 2010;19(6):357-359
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of proteasome inhibitor bortezomib (BOR) on proliferation inhibition and apoptosis in imatinib-resistant chronic myeloid leukemia cell line K562/G01. Methods MTT assay was used to observe the effect of growth inhibitory of cells; flow cytometry was used to detect cell cycle and apoptosis. Results K562/G01 cells was not sensitive to imatinib,1C50 values of imatinib to K562 and K562/G01 cells was (20.09±1.38) and (0.54±0.13) μmol/L,respectively; BOR could inhibit proliferation in K562/G01 cell,peaked at 48 h,and IC50 value of BOR to K562/G01 was (23.10±2.71) nmol/L. G2/M phase cell cycle arrest and significant apoptosis peak could be seen by flow cytometry with increased in the concentration and action time of BOR. Conclusion BOR can inhibit proliferation and induce apoptosis in imatinib-resistant K562/G01 cell,the mechanism may be related to cell cycle G2/M phase arrest.