Utility of fluorescence in situ hybridization in the deletion of chromosome 13 in multiple myeloma and its clinical significance

VernacularTitle:荧光原位杂交技术在多发性骨髓瘤患者del(13q14)检测中的应用
Author: Jufen XIE ; Yongan ZHOU ; Liping SU ; Kai WANG ; Jin ZHAO ; Li MA
Publication Type:Journal Article
Keywords: Multiple myeloma; In situ hybridization; fluorescence; del(13q14); Retinoblastoma-1 gene
From: Journal of Leukemia & Lymphoma 2009;18(6):338-341
CountryChina
Language:Chinese
Abstract: Objective To explore the deletion of chromosome 13 in multiple myeloma (MM), clinical significance of FISH-defined partial deletion chromosome 13 in MM patients were investigate. Methods Fluorescence in situ hybridization (FISH ) was performed on bone marrow from 38 patients with MM to study the deletion of Rb-1 gene and locus 13q14 on chromosome 13. Fisher exact propability was used to study the relations between partial deletion of chromosome 13 and clinical features. Results 20 out of the 38 cases were found with deletion of chromosome 13; deletion of Rb-1 gene in 4 cases; deletion of locus 13q14 in 2 out of 38 cases; and 14 cases with both of deletions. Fisher exact propability showed that deletion of chromosome 13 was associated with hypso-serum lactic dehydrogenase, stage of ISS. Conclusion Deletion of Rb-1 gene and locus 13q14 were both common cytogenetic changes in MM patients with effect on the biological behavior of the disease, but the value of del (13q14) in MM needs further investigation. FISH was a rapid, accurate and sensitive technique in the analysis of del (13q14) in MM.