Application of multiplex reverse transcription polymeruse chain reaction in acute myeloid leukemia
10.3760/cma.j.issn.1009-9921.2009.05.008
- VernacularTitle:多重巢式RT-PCR技术在急性髓系白血病中的应用
- Author:
Jin ZHAO
;
Yongan ZHOU
;
Liping SU
;
Jianrui WU
;
Kai WANG
;
Jufen XIE
;
Li MA
- Publication Type:Journal Article
- Keywords:
Acute myeloid leukemia;
Chromosomal structural aberration;
Fusion gene;
Multiplex RT-PCR
- From:
Journal of Leukemia & Lymphoma
2009;18(5):277-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the fusion genes derived from chromosome structural aberrations in acute myeloid leukemia(AML) and the relationship between fusion genes and the MICM classification, clinical diagnosis, chemotherapy and prognosis. Methods The expression of fusion gene in bone marrow samples was detected with multiplex RT-PCR technique and chromosome karyotypes, immunological phenotypes and clinical data were analyzed in 60 acute myeloid leukemia newly diagnosed. Results 37 cases(61.67 %) of 60 patients carried 5 kinds of fusion genes consisting of MLL-AF9, TLS-ERG, CBFβ-MYH1, AML1-ETO and PML-RARα. The activation of oncogene HOX11 was detected in 13 AML cases, three of them with other chromosome aberration simultaneously.23 cases of 31 patients carrying AML1-ETO or PML-RARα, reached complete remission(CR) after chemotherapy and without relapse. Conclusion Gene typing is the most precise classification method that can direct clinical treatment and evaluate prognosis. Multiplex RT-PCR technique, which can quickly screen 29 kinds of fusion gene derived from chromosome structural aberrations at one time, maybe helpful to improve M1CM classification and guide the choice of treatment.
