Effect of Electro-Acupuncture on S-100 Protein Expression of Nerve Fibers in the Repair Process of Transected Sciatic Nerve in Rats
- VernacularTitle:Effect of Electro-Acupuncture on S-100 Protein Expression of Nerve Fibers in the Repair Process of Transected Sciatic Nerve in Rats
- Author:
Qingwen LI
- Publication Type:Journal Article
- Keywords:
Electro-acupuncture;
Nerve Fibers/Injuries;
S-100 protein
- From:
Journal of Acupuncture and Tuina Science
2007;5(1):10-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-acupuncture treatment for peripheral nerve injury.Methods: Following rat's sciatic nerve transection, the nerve regeneration chamber model was established, then the rat was given electro-acupuncture stimulation at the frequency of 5 Hz and frequency of 100 Hz, and the immunohistochemical staining method and image analyzing semi-quantitative technique were adopted to determine the percentage of S-100 protein area,hence to observethe effect of electro-acupuncture on the repair process of transected sciatic nerve of rat and the S-100 protein expression. Results: Electro-acupuncture could significantly potentiate the S-100 protein expression of Schwann Cells (SC) in the repair process of transected sciatic nerve. At the 14th week, 17th week and 20th week after operation, the percentages of S-100 protein area were more obviously increased in 5 Hz electro-acupuncture group and 100 Hz electro-acupuncture group than in model group, with significant differences (P＜0.05). Especially, the better effect was obtained in 5 Hz electro-acupuncture group. Judging from the dynamic treatment, it is very vital to be treated early. Conclusion: Electro-acupuncture is an effective method for the treatment of peripheral nerve injury, and its therapeutic effect is related to the improvement of SC proliferation.
