Study on optimized conditions for cord blood CD+34 cells amplification in vitro

VernacularTitle:脐带血CD+34细胞体外扩增优化条件的研究
Author: Yuming ZHANG ; Liming JIANG ; Dan XIONG ; Qinghua LI ; Zhigang YANG
Publication Type:Journal Article
Keywords: Fetal blood; CD+34cells; Proliferation; Feeder-layers
From: Journal of Leukemia & Lymphoma 2009;18(4):203-205
CountryChina
Language:Chinese
Abstract: Objective To set up the optimized conditions,the amplification of eord blood CDCD+34 cells in vitro were analyzed by comparing the conditions such as different feeder-layers, stimulating-factors or purity/contents of those cells. Methods The cord blood CDCD+34 cells proliferation was analyzed by the methods of MTT, cell counting, and flow eytometer. The amplification and clone-forming ability of cord blood CDCD+34 cells were detected under optimized condition. Resulits The growth rates of cord blood CD+34 cell under optimized conditions(10 times) were significantly higher than that of the control(2.8 times) (P ＜0.01), and the cloneforming ability of cord blood CDCD+34 cells under optimized conditions(CFU-C 36.67±6.11) were also better than that of the control(CFU-C 16.33±1.53) (P ＜0.01). Conclusion The cord blood CD+34 cells proliferation can be promoted in the co-cultured system, and the character of the stem cells were kept well in that system.