Simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture by high performance liquid chromatography
10.1016/j.jpha.2011.06.006
- Author:
Zhisheng XIE
;
Xinjun XU
;
Chunyan XIE
;
Jieyun HUANG
;
Mei YANG
;
Ruiming LI
;
Xiao CHEN
- Publication Type:Journal Article
- Keywords:
12,13-Dihydroxy-euparin;
Glycyrrhizic acid;
Yanyanfang mixture;
High performance liquid chromatography
- From:
Journal of Pharmaceutical Analysis
2011;01(3):219-222
- CountryChina
- Language:Chinese
-
Abstract:
A reversed phase high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 12,13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.A Grace Apollo C18 column (250 mm× 4.6 mm,5 μm) was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid (0.2％,v/v).Gradient elution was carried out at the flow rate of 1.0 mL/min and the column temperature was 30 ℃.An ultraviolet (UV) detector was used with a selected wavelength of 240 nm.Calibration curves were linear within the concentration range of 4.6-45.75 μg/mL for 12,13-dihydroxyeuparin (r＞0.9999) and 106.9-1068.9μg/mL for glycyrrhizic acid (r＞0.9999),respectively.Recoveries were 102.18 ％ for 12,13-dihydroxyeuparin and 101.17％ for glycyrrhizic acid.The method developed could be applied to the simultaneous determination of 12,13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.
