Detection of the effect of bortezomib with arsenic trioxide different concentration on the cell cycle and apoptosis of Raji cells by flow cytometry
10.3760/cma.j.issn.1009-9921.2010.12.006
- VernacularTitle:流式细胞术检测亚砷酸、硼替佐米对淋巴瘤细胞株细胞周期和凋亡率的影响
- Author:
Xiujuan ZHAO
;
Yuliang SHI
;
Ling XIAO
;
Chengjin YAN
;
Lihua MENG
;
Zhilin GAO
;
Hongyan JIN
;
Xinlei GUO
- Publication Type:Journal Article
- Keywords:
Lymphoma;
Flow cytometry;
Arsenic trioxide;
Bortezomib;
Cell cycle;
Apoptosis
- From:
Journal of Leukemia & Lymphoma
2010;19(12):721-723
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of bortezomib with arsenic trioxide different concentration on the cell cycle and apoptosis of Raji cells. Methods Flow cytometry analysis showed that the relative number of cells in different phases and the percentages of cells calculated in G1 and S phase of the cell cycle and apoptosis were assessed after treatment with As2O3 and BOR or in combination with BOR in different concentration at indicated time (24 h, 48 h, 72 h). Results Flow cytometric analysis showed that the cell cycle was arrested at G1 phase, the number of cells G1 period increased significantly, and S phase decreased on Raji cells after As2O3 treatment. The relationship between the cellular DNA contents and the concentration of As2O3 showed a dose-and time-dependent manner (P ＜0.0001). But it was found that BOR had no effect on Raji cell cycle, but, in two drugs combination, cell apoptosis rate significantly increased from 16.98 % to 45.84 %. Conclusion The results show that As2O3 exerted variable and definite effects on lymphoma Raji cells, which indicated that As2O3 might induce apoptosis and arrest cell cycle. The combination of two drugs had a effective and synergistic effect on apoptosis.
