Effects on cellular immunity caused by intravenous infusion of allogenic rhesus mesenchymal stem cells
10.3760/cma.j.issn.1009-9921.2011.09.012
- VernacularTitle:骨髓间充质干细胞静脉输注对猕猴细胞免疫功能的影响
- Author:
Chuanbo FAN
;
Zhaohui WANG
;
Lei WANG
;
Kaixun HU
;
Lihui LIU
;
Qiyun SUN
;
Li BIAN
;
Qingchao WU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Macaca;
Immunity
- From:
Journal of Leukemia & Lymphoma
2011;20(9):550-553
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the changes of cellular immunity caused by intravenous infusion of allogenic rhesus mesenchymal stem cells (MSCs).MethodsMSCs were isolated and cultured.Then the immunomodulatory effects after MSCs infusion were evaluated by means of peripheral blood counts,mixed lymphocyte reaction (MLR) and analysis of lymphocytic subgroup. ResultsMSCs of rehsus were successfully cultivated. No acute toxicities or GVHD were observed in recipients. No obvious changes of peripheral blood counts were present. Recipients A2, A3, A4 were administered with MSC by 4.0 ×105/kg, 1.0 ×106/kg, 2.0×106/kg respectively and relative reaction (RR) of MLR decreased 14 days post MSCs infusion: from 46±2.6 ％to 40.4±1.73 ％ (F =10.19, P =0.023), from (40.9±2.3) ％ to (33±2.1) ％ (F =2.593, P =0.013), from 48.3±2.0 ％ to 39±1.0 ％ (F =28.431, P =0.003) respectively. The decrease degree (ARR) was positively related to the amount of MSCs(F =27.413, P =0.038). RR was restored within 30 days post MSCs infusion. After MSCs infusion, CD3+ CD3+CD4+ and CD3+CD8+ T-lymphocytes decreased in recipient A4, who was administered with the largest number of MSCs, and restored within 30 days. ConclusionMSCs infusion without any other treatment could temporarily inhibit immunity of T lymphocytes in MLR and the immunity inhibition was positively related to the amount of MSCs.The specific immunological characteristics of MSCs were demonstrated with extensive prospect in clinical research.
