Effect of intramyocardial controlled releasing base fibroblast growth factor stent combined with bone marrow-derived mesenchymal stem cells transplantation on cardiac repair in porcine acute myocardial infarction models
- VernacularTitle:碱性成纤维细胞生长因子缓释支架联合骨髓间质干细胞治疗猪心肌梗死的心肌SPECT显像
- Author:
Xiaobin ZHAO
;
Rongfang SHI
;
Kun TANG
;
Jian ZHAO
;
Ying WANG
;
Xiaocheng LIU
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Base fibroblast growth factor;
Tomography,emission-computed,single photon;
Porcine
- From:
Chinese Journal of Medical Imaging Technology
2010;26(3):410-413
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the therapeutic effect of intramyocardial controlled releasing base fibroblast growth factor (b-FGF) stent combined with transplanting bone marrow-derived mesenchymal stem cells (BM-MSCs) in porcine acute myocardial infarction (AMI) models. Methods Eighteen porcines were divided into three groups (each n=6):transmyocardial revascularization (TMR)+naked stent (control group), TMR+b-FGF stent (b-FGF group) and TMR+b-FGF stent+BM-MSCs (b-FGF+BM-MSCs group). Acute myocardial infarction models were induced by ligating the left anterior descending artery in 18 porcine. In the control and b-FGF group, naked stents, b-FGF stents and b-FGF stent+BM-MSCs were implanted into TMR channels in the infarct and border zone. ~(99m)Tc-methoxyisobutylisonitrile (MIBI) myocardial perfusion imaging were performed to evaluate changes in myocardial blood flow, and echocardiography and immunohistochemical studies were also performed. Results After 6 weeks, the quality of myocardial infarction, fractional shortening, neovascular density in b-FGF and b-FGF stent+BM-MSCs group were higher than those of control gruop (P<0.05), and in b-FGF stent+BM-MSCs group was higher than those in b-FGF group (P<0.05). Conclusion BM-MSCs combined with controlled releasing intramyocardial b-FGF stent can improve the flow of myocardial infarction area, promote angiogenesis and heart function, and the therapeutic effect which can be evaluated with myocardial SPECT imaging.
