Effect of lipopolysaccharide on the iron metabolism in macrophages

VernacularTitle:脂多糖对巨噬细胞铁代谢的影响
Author: Li WANG ; Baohua JIANG ; Zhongming QIAN ; Xianglin DUAN
Publication Type:Journal Article
Keywords: Divalent metal transporter 1; Ferroportin 1; Primary culture; Macrophage; Mouse
From: Acta Anatomica Sinica 2014;(5):656-662
CountryChina
Language:Chinese
Abstract: Objective To study the effect of lipopolysaccharide ( LPS ) on the activity of primary cultured macrophages and the distribution of divalent metal transporter 1 ( DMT1 ) and ferroportin 1 ( FPN1 ) .Methods Primary cell culture , MTT chromotest , cytochemistry chromotest and cell immunofluorescence techniques were used in this work . Results DMT1 was mainly distributed in the cytoplasm , which illuminates that DMT1 mediates the macrophage intracellular transit of iron from phagolysosome to cytoplasm .FPN1 was distributed in the cytoplasm and membrane , and the cytoplasm was the main site of FPN 1 distribution in macrophages .Conclusion Iron liberation from heme inside the phagolysosome occurs after erythrophagocytosis and it is possible that FPN 1 mediates intracellular transit of iron released by heme catabolism .The study found that LPS promoted the cell growth and this effect was reached to the peak in the 10 -5μg/L LPS group, but the cell growth was blocked with the increase of LPS concentration .