Construction of tissue engineered adipose using human adipose stem cells with chitosan-modified silk fibroin
10.3969/j.issn.2095-4344.2014.39.016
- VernacularTitle:壳聚糖修饰丝素蛋白与人脂肪间充质干细胞体外构建组织工程脂肪
- Author:
Ting KANG
;
Gang WANG
;
Yi LIU
;
Gangqiang LIU
- Publication Type:Journal Article
- Keywords:
chitosan;
silk fibroin;
mesenchymal stem cells
- From:
Chinese Journal of Tissue Engineering Research
2014;(39):6323-6328
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Based on the original advantages of silk fibroin, positive charged water-soluble chitosan modified silk fibroin is modified on surface and could improve celladhesion on the scaffolds. OBJECTIVE:To verify the biocompatibility of chitosan-modified silk fibroin with human adipose-derived stem cells (hADSCs), and feasibility of constructing tissue engineered adipose in vitro. METHODS:The hADSCs at passage 3 were seeded on chitosan-modified silk fibroin at the concentration of 1×107/L, as the experiment group;at the same cellconcentration, hADSCs were seeded in 96-wel plates as the control group. MTT tests were performed to evaluate the adhesion, growth and proliferation of hADSCs on chitosan-modified silk fibroin. Then hADSCs were implanted on the chitosan-modified silk fibroin scaffolds at the concentration of 1×109/L. The hADSCs seeded onto chitosan-modified silk fibroin complexes were respectively cultured with adipogenic differentiation medium and ordinary high-glucose DMEM. The complexes were stained with oil red O, and detected with RT-PCR after cultured 14 days. RESULTS AND CONCLUSION:The hADSCs adhered to and proliferated on the scaffolds. After cultured with adipogenic differentiation medium for 14 days, oil red O staining demonstrated that there were amount of mature adipocytes on the scaffold. The peroxisome proliferator activated receptorγ2 was positively expressed. The chitosan-modified silk fibroin possessed excellent biocompatibility in vitro. The co-cultured hADSCs could be induced to mature adipocytes successful y.
