Impact of different preparation methods for graft materials on biological properties of allogeneic tendon
10.3969/j.issn.2095-4344.2014.39.020
- VernacularTitle:不同方法制备移植修复材料对异体肌腱生物学性能的影响
- Author:
Hongxing ZHANG
;
Geng LIU
;
Wuan QIU
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
biomechanics;
transplantation
- From:
Chinese Journal of Tissue Engineering Research
2014;(39):6348-6352
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The transplanted tendon must have good biomechanical properties, in order to effectively avoid tendon tear at the anastomosis end during suturing and reduce adhesion of tendon during healing process. OBJECTIVE:To investigate the effects of different methods for preparation of graft materials on the biological properties of tendon al ograft. METHODS:Forty-eight healthy male Leghorns were randomly divided into three groups:vitrification group, chemical extraction group, and control group. Unilateral superficial and deep flexor tendon of the third toe was subjected to vitrification, chemical extraction and no treatment in the three groups, respectively. A part of tendon was taken for biomechanical testing, and the other part was for al ogeneic transplantation. After 1, 2, 3, 6 weeks, peripheral blood CD4+, CD8+T lymphocytes were counted. RESULTS AND CONCLUSION:Vitrification could partial y retain the original tendon cells, but the chemical extraction method could not. Tensile strength for tendon rupture, tensile fracture power and tensile elongation at break were not statistical y significant among three groups (P>0.05). At the end of 1 and 2 weeks after transplantation, CD4+, CD8+, CD4+/CD8+difference was significant among the three groups (P<0.05);at the end of 3 and 6 weeks after transplantation, CD4+, CD8+, CD4+/CD8+were significantly less in the vitrification and chemical extraction groups than the control group (P<0.05), but no difference was found between the vitrification group and chemical extraction group (P>0.05). These findings indicate that the vitrification and chemical extraction methods can significantly reduce immunogenicity of the tendon based on effective retention of biomechanical properties of the tendon.
