Adipose-derived mesenchymal stem cells differentiate into inner ear hair cells in guinea pigs induced by progressive addition of cytokines
10.3969/j.issn.2095-4344.2014.37.017
- VernacularTitle:分阶段添加细胞诱导因子诱导豚鼠脂肪间充质干细胞定向分化内耳毛细胞
- Author:
Xiaoyan WANG
;
Bingbing LI
;
Enfeng ZHANG
;
Xiaojuan BI
;
Lizhong LIU
- Publication Type:Journal Article
- Keywords:
adipose tissue;
mesenchymal stem cells;
guinea pigs;
hair cells,auditory;
cytokines
- From:
Chinese Journal of Tissue Engineering Research
2014;(37):5998-6002
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Sensorineural hearing loss is mainly caused by missing or damaged hair cells in the inner ear. Application of adipose-derived mesenchymal stem cells to regenerate inner ear hair cells is an effective treatment for hearing loss. OBJECTIVE:To explore the feasibility of in vitro inducing adipose-derived mesenchymal stem cells to differentiate into inner ear hair cel-like cells in guinea pigs. METHODS:Adipose-derived mesenchymal stem cells from guinea pigs were isolated and cultured to the 3rd generation. cellphenotype was detected using flow cytometry. Cytokines were added for induction and differentiation by stages, including epidermal growth factor, basic fibroblast growth factor, insulin-like growth factor-1, al-trans retinoic acid, brain-derived neurotrophic factor, neurotrophin 3. RESULTS AND CONCLUSION:Adipose-derived mesenchymal stem cells of guinea pigs cultured in vitro were fusiform and showed a swirled adherent growth. Passage 3 cells were positive for CDCD29 and CD44, but negative for CD34 and CD45. After induction, the cells were positive for nestin and GFAP positive at early stage;after 10-day continuous induction, the cells expressed Myosin VIIa and Math1, specific markers of hair cells, indicating that cytokines can directly induce adipose-derived mesenchymal stem cells to differentiating into inner ear hair cells in guinea pigs.
