Effects of basic fibroblast growth factor via coronary venous retroperfusion on bone marrow mesenchymal stem cell differentiation in vivo
10.3969/j.issn.2095-4344.2014.37.003
- VernacularTitle:经冠状静脉逆行灌注碱性成纤维细胞生长因子对骨髓间充质干细胞体内分化的影响
- Author:
Xiao WANG
;
Lei ZHEN
;
Huangtai MIAO
;
Xingxin WU
;
Hongmei REN
;
Shutian SHI
;
Yan QIAO
;
Xinmin LIU
;
Bin QUE
;
Shaoping NIE
- Publication Type:Journal Article
- Keywords:
bone marrow;
mesenchymal stem celltransplantation;
fibroblast growth factor 2;
myocardial infarction
- From:
Chinese Journal of Tissue Engineering Research
2014;(37):5916-5922
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In vitro studies have demonstrated that basic fibroblast growth factor (bFGF) promote the differentiation of bone marrow mesenchymal stem cells (BMSCs) into cardiomyocyte-like cells. However, it is unclear whether coronary venous retroperfusion of bFGF stimulates BMSCs differentiation in vivo. OBJECTIVE:To evaluate the effects of coronary venous retroperfusion of bFGF on BMSCs differentiation in vivo. METHODS:BMSCs from 12 dogs were isolated by density gradient centrifugation and expanded in vitro. These cells were transfected by enhanced green fluorescence protein (EGFP) lentiviral vector and the transfection efficiency was analyzed. Acute myocardial infarction was induced by ligation of left anterior descending coronary artery. After 1 week, 10 survival animals were randomized to BMSCs group (n=5) and bFGF+BMSCs group (n=5). bFGF-and EGFP-positive BMSCs were reversely infused via coronary vein using over-the-wire bal oon catheter. One week after infusion, the number of EGFP-positive cells co-staining factor VIII and troponin I was compared between the two groups by immunofluorescence method. RESULTS AND CONCLUSION:BMSCs were successful y transfected by EGFP and the transfection efficiency was 85%. Immunofluorescence showed that EGFP-positive BMSCs were observed in 23.5%of slides. There were more EGFP-positive cells co-staining VIII and troponin I in the bFGF+BMSCs group than in the BMSCs group (P<0.05). Thus, the coronary venous retroperfusion of bFGF enhances the differentiation of BMSCs into vascular endothelial cells and cardiomyocytes. Combined delivery of bFGF and BMSCs can exert a synergistic effect to promote cardiac repair.
