Meropenem effects on biological characteristics of umbilical cord-derived mesenchymal stem cells during umbilical cord collection
10.3969/j.issn.2095-4344.2014.37.007
- VernacularTitle:脐带采集液中添加美罗培南对脐带间充质干细胞生物学特性的影响
- Author:
Junjiang LIU
;
Jianyu ZHOU
;
Wenjing HUANG
;
Jingxin HONG
- Publication Type:Journal Article
- Keywords:
umbilical cord;
mesenchymal stem cells;
beta-lactams;
anti-bacterial agents
- From:
Chinese Journal of Tissue Engineering Research
2014;(37):5942-5946
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Due to the difficulty in the control of delivery and col ection process, antibiotics are often added into the preservation fluid in order to avoid bacterial contamination but not affect cellgrowth and proliferation. OBJECTIVE:To observe the effects of meropenem on the proliferation and differentiation potential of umbilical cord-derived mesenchymal stem cells. METHODS:There were two groups in this experiment:control group, preservation fluid with penicil in-streptomycin (final concentration of 100 U/mL);experimental group, preservation fluid with meropenem (final mass concentration of 1.0 mg/L). 100 umbilical cord samples were col ected in each group, and the positive rate was calculated. After isolation and culture, the passage 3 cells were used to draw a growth curve, flow cytometry analysis was used for phenotype determination, and osteogenic and adipogenic differentiation of cells were detected. RESULTS AND CONCLUSION:The contamination rates were 3%(3/100) in the experimental group and 20%(20/100) in the control group, indicating that meropenem can obviously reduce the contamination rate. In the experimental group, the morphology, differentiation potential and cellphenotype of the passage 3 cells were al normal. The proliferation ability of cells showed no difference between the two groups. Therefore, meropenem can be added to the preservation fluid.
