Co-culture of adipose-derived stem cells and osteoblasts under different conditions
10.3969/j.issn.2095-4344.2014.37.018
- VernacularTitle:不同培养条件下脂肪干细胞与成骨细胞的共培养
- Author:
Yang ZHANG
;
Dacheng LIU
;
Xiaoning YANG
- Publication Type:Journal Article
- Keywords:
adipocytes;
osteoblasts;
celldifferentiation;
cellculture techniques
- From:
Chinese Journal of Tissue Engineering Research
2014;(37):6003-6007
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:After co-culture with osteoblasts, bone marrow stem cells can be induced to differentiate into osteoblasts. Whether adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts or not? OBJECTIVE:To observe whether adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts. METHODS:Adipose-derived stem cells and osteoblasts were isolated from New Zealand white rabbits. Then, passage 3 adipose-derived stem cells were co-cultured with passage 2 osteoblasts in 10%or 5%fetal bovine serum for 14 days. RESULTS AND CONCLUSION:After 7 days of co-culture, some adipose-derived stem cells became round in the two groups. After 14 days of co-culture, adipose-derived stem cells highly differentiated and differentiated cells were similar to mature osteoblasts that were positive for alkaline phosphatase staining and alizarin red staining. The mRNA expression of type I col agen and osteocalcin increased in both two group, especial y in the 10%fetal bovine serum group. These findings indicate that adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts induced by high-concentration serum culture.
