Amniotic cells protect and repair mouse brain cells following ischemia-reperfusion injury
10.3969/j.issn.2095-4344.2014.37.022
- VernacularTitle:羊膜细胞可保护和修复缺血再灌注损伤小鼠脑组织细胞
- Author:
Yantao ZHENG
;
Bin LIU
;
Lodato ROBERT
;
Qilin LI
;
Dihui LAN
;
Xiaoying HONG
;
Hua XIAN
- Publication Type:Journal Article
- Keywords:
amnion;
reperfusion injury;
cellcycle;
flow cytometry
- From:
Chinese Journal of Tissue Engineering Research
2014;(37):6024-6028
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Amniotic cells are mainly composed of amniotic epithelial cells and amniotic mesenchymal cells, which have multi-differentiation potential and can be transformed into neurons as wel as synthesize and release biological y active substances and neurotrophic factors. In preliminary studies, amniotic cells that are transplanted into the brain can significantly promote the regeneration of brain neurons. OBJECTIVE:To explore the role of amniotic cells in mouse brain cells after ischemia-reperfusion injury. METHODS:The model of cerebral ischemia-reperfusion injury was established in Babl/c mice using occlusion of bilateral common carotid arteries, and then brain cells were separated from mice. Amniotic cells were isolated from mouse placenta. Brain cells from Balb/C mice co-cultured with amniotic cells served as experimental group, and brain cells cultured with PBS as control group. RESULTS AND CONCLUSION:The viability of brain cells in the experimental group was significantly higher than that in the control group (P<0.05). There was no difference in necrotic rate of brain cells between the experimental and control groups after 24 and 72 hours co-culture (P>0.05);after 48 hours co-culture, however, the necrotic rate of brain cells was significantly lower in the experimental group than the control group (P<0.05). In cellcycle, the experiment group showed increased S phase cells;while, the control group exhibited increased G 1 phase cells and decreased S phase cells. G 2 phase cells had no changes in number in both two groups. Through the above results, amnion cells can be proved to protect and promote the regeneration of brain cells of Balb/C mice with ischemia-reperfusion injury, and inhibit cellnecrosis and apoptosis.
