Research status on Ts-SV40-mediated temperature sensitive cells
10.3969/j.issn.0253-9896.2015.03.027
- VernacularTitle:Ts-SV40介导的温敏细胞研究现状
- Author:
Yuping LIAO
;
Xiaohong LI
;
Hongtao SUN
- Publication Type:Journal Article
- Keywords:
transfection;
hypothermia,induced;
review;
ts-SV40;
temperature sensitive cells;
hypothermia;
immortal-ization
- From:
Tianjin Medical Journal
2015;(3):323-326
- CountryChina
- Language:Chinese
-
Abstract:
The in vitro passage number and proliferation of non-immortalized cells are limited, which restrictions cell therapy or in vitro studies. Cells transfected with temperature sensitive simian virus 40 large T antigen (ts-SV40LT) gene could show the greatest proliferation. The cells can be amplified with compensating the lack of limited number of cells under the permissive temperature. Non-permissive temperature can be used in studying the cell therapy or its other physiological characteristics. This research field involves peritoneal stromal cells, satellite cells of urinary tract, oral epithelial cells, adre?nal medullary cells, bone marrow-derived endothelial cells, retinal progenitor cells, mesenchymal stem cells, hematopoietic stem cells, mast cells, podocytes and Kupffer cells. In this study, the current research on Ts-SV40-mediated temperature-sensitive cells was reviewed.
