Effect of thrombopoietin on chemical hypoxia-induced apoptosis of PC12 cells
10.3969/j.issn.1000-4718.2015.03.005
- VernacularTitle:促血小板生成素对化学性缺氧诱导的P C12细胞凋亡的影响
- Author:
Xiaodong WANG
;
Meiyuan CHEN
;
Zhi ZENG
;
Zhuoyan ZHOU
;
Mo YANG
- Publication Type:Journal Article
- Keywords:
Thrombopoietin;
Cobalt chloride;
PC12 cells;
Cell hypoxia;
Cell apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(3):409-414
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To study the effect of thrombopoietin ( TPO) on chemical hypoxia-induced apoptosis of the Rattus norvegicus adrenal pheochromocytoma (PC12) cells.METHODS:The cultured PC12 cells were randomly divided into normal control group, cobalt chloride ( CoCl2 ) group, CoCl2 +TPO group and TPO group.The cell viability was mea-sured by MTT assay.The effect of TPO on CoCl2-induced cell apoptosis was analyzed by flow cytometry with Annexin V/PI double staining.The intracellular reactive oxygen species ( ROS) were detected by fluorescence microscopy, and the chan-ges of the mitochondrial membrane potential ( MMT) were determined by flow cytometry and fluorescence microscopy.RE-SULTS:Chemical oxygen agent CoCl2 significantly inhibited the growth of PC12 cells (P<0.01).The apoptotic rate in CoCl2 group was obviously higher than that in control group ( P<0.05) , while the apoptotic rate in CoCl2 +TPO group was obviously lower than that in CoCl2 group (P<0.05).TPO decreased the production of ROS, and inhibited the decrease in MMP induced by CoCl2(P<0.01).CONCLUSION: TPO has a protective effect against CoCl2-induced apoptosis of PC12 cells by decreasing the production of ROS and inhibiting the decrease in MMP.
