Generation of induced pluripotent stem cells and neural cells from urine-derived cells of Alzheimer disease patients

VernacularTitle:阿尔茨海默病患者尿液细胞向诱导多能干细胞及神经细胞的诱导转化
Author: Rui WEI ; Zhong LI ; Xiujuan CAI ; Lu HE ; Qingfeng LEI
Publication Type:Journal Article
Keywords: Alzheimer disease; Induced pluripotent stem cells; Neural differentiation
From: Chinese Journal of Pathophysiology 2015;(3):421-427
CountryChina
Language:Chinese
Abstract: [ ABSTRACT] AIM:In this study, we aim to obtain the induced pluripotent stem cells ( iPSCs) from the patients with sporadic Alzheimer disease ( AD) .METHODS:Three typical Alzheimer’ s patients were chosen, and the epithelial cells were isolated from their urine.We reprogrammed these cells into induced pluripotent stem cells by transfection of 4 factors (Oct4, Sox2, Klf4 and SV40LT) with the technique of electro-transfection.After getting these iPSCs, we continue to differentiate them into neural cells by a specific method—dual inhibition of Smad signaling.RESULTS: The primary cells from 3 AD patients were successfully reprogrammed to iPSCs, and these patients-derived iPSCs were differentiated into neural cells.There was no significant difference, during iPSCs reprogramming and neural differentiation, between cells from AD patients and normal people.CONCLUSION: The urine cells from AD patients were able to transfer to iPSCs, functional neurons and neurogliocytes.