Knockdown of SALL4 expression regulates cell proliferation and apoptosis in prostate cancer LNCaP cells
10.3969/j.issn.1000-4718.2015.03.009
- VernacularTitle:沉默婆罗双树样基因4对前列腺癌 LNCaP 细胞增殖和凋亡生物学行为的影响
- Author:
Jin LI
;
Hai HUANG
;
Yiming LAI
;
Lexiang ZENG
;
Yi CAO
;
Ganping WANG
;
Xianju CHEN
;
Yongsheng YU
;
Jieqing CHEN
;
Simin ZHANG
;
Yiming ZHANG
;
Zhenghui GUO
- Publication Type:Journal Article
- Keywords:
SALL4 protein;
Prostate neoplasms;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(3):435-439
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM: To investigate the SALL4 expression, proliferation and apoptosis in the LNCaP cells after transfection of SALL4 siRNA.METHODS: The expression of SALL4 at mRNA and protein levels was detected by real-time PCR and Western blotting.MTS assay, colony formation assay and flow cytometry were used to determine the prolifer-ation, colony formation ability and apoptosis of the LNCaP cells.The effect of SALL4 on the expression of Bax and Bcl-2 was analyzed by Western blotting.RESULTS:Compared with negative control group, the expression of SALL4 at mRNA and protein levels in LNCaP cells was down-regulated by transfection of SALL4 siRNA ( P<0.05 ) .The proliferation rate and colony formation ability were decreased, while apoptosis rate increased in si-SALL4 group (P<0.05).Higher expres-sion of Bax and lower expression of Bcl-2 in si-SALL4 group were observed ( P<0.05 ) .CONCLUSION:Down-regula-tion of SALL4 by siRNA not only suppresses LNCaP cell proliferation and colony formation, but also inhibits Bcl-2 expres-sion and activates Bax expression to induce apoptosis.
