miR-486-5p enhances senescence of human mesenchymal stem cells by de-creasing telomerase activity
10.3969/j.issn.1000-4718.2015.03.029
- VernacularTitle:miR-486-5p 通过抑制端粒酶活性促进人骨髓间充质干细胞衰老
- Author:
Jiao LI
;
Jingzhi ZHANG
;
Ningning LIU
;
Bohang ZENG
;
Jun DONG
;
Shiming LIU
- Publication Type:Journal Article
- Keywords:
Human mesenchymal stem cells;
MicroRNA-486-5p;
Senescence
- From:
Chinese Journal of Pathophysiology
2015;(3):547-551
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of microRNA-486-5p (miR-486-5p) on the senescence of human mesenchymal stem cells ( hMSCs).METHODS: The expression of miR-486-5p was determined by miRNA arrays and real-time PCR.By transfection of miR-486-5p mimic or inhibitor, up-regulation or down-regulation of miR-486-5p expres-sion in hMSCs was established.The effect of miR-486-5p and silence information regulator 1 (SIRT1) on hMSC telomerase activity and senescence were detected byβ-galactosidase staining.RESULTS:The expression of miR-486-5p was up-regu-lated in the old hMSCs compared with the young hMSCs.Up-regulation of miR-486-5p resulted in increasing senescence of hMSCs.Conversely, down-regulation of miR-486-5p resulted in decreasing cell senescence.The expression of SIRT1 and telomerase reverse transcriptase ( TERT) was down-regulated in the old hMSCs compared with the young hMSCs.Directly repression of SIRT1 expression inhibited the hMSC TERT protein expression and telomerase activity, but increased cell se-nescence.The regulation of miR-486-5p on hMSC senescence was attenuated by inhibiting the expression of miR-486-5p and SIRT1 together.CONCLUSION:miR-486-5p enhances senescence of hMSCs by decreasing the expression of SIRT1 and telomerase activity.
