Expression of Gab2 in human osteosarcoma U2-OS cells
10.3969/j.issn.1000-4718.2015.03.033
- VernacularTitle:Gab2在人骨肉瘤细胞U2-OS中的表达及意义
- Author:
Yong PING
;
Pingshan DI
;
Weibing LI
;
Fengmei LI
;
Weiyi CHEN
;
Pingping CHEN
- Publication Type:Journal Article
- Keywords:
Grb2-associated binding protein 2;
Small interference RNA;
Cell invasion;
U2-OS cells
- From:
Chinese Journal of Pathophysiology
2015;(3):568-571
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteo-sarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells.METHODS: The tech-nique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines.Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells.After transfection, through chem-otaxis and invasion assays in vitro, the cell migration and invasion abilities were detected.RESULTS:After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells ( SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells ( Scr/U2-OS ) and U2-OS cells.After stimulation with epidermal growth factor ( EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells ( P<0.01 ) .The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups ( P<0.01) .CONCLUSION:Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line.
