Construction of novel 3-D composite bionic network and evaluation of its histocompatibility
10.3969/j.issn.0253-9896.2015.03.003
- VernacularTitle:新型三维复合仿生网络的构建及其组织相容性评价
- Author:
Man CUI
;
Xin ZHANG
;
Litao MA
;
Pengcheng CHE
;
Fanglian YAO
;
Hong SUN
- Publication Type:Journal Article
- Keywords:
neovascularization,physiologic;
histocompatibility;
tissue engineering;
chitosan;
3-D composite bionic net-work
- From:
Tianjin Medical Journal
2015;(3):233-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct novel 3-D composite bionic network and evaluate the histocompatibility . Meth?ods The novel 3-D composite bionic network was prepared from chitosan, hydroxyapatite, gelatin and pectin in certain ra?tio by biomimtic approach, which was co-cultured with MC3T3-E1. The cell compatibility was studied by using inverted phase contrast microscope, routine paraffin section staining, scanning electron microscopy and F-DA staining. The resultant scaffold material was implanted into the dorsal subcutaneous space of SD rats. The histocompatibility, blood vessel capabili?ties and the degradation of the material were observed 2, 4, 8 and 12 weeks after surgery. Results The structure of novel 3-D composite bionic network was three-dimensional and porous. The cells attached on scaffolds attached and grew well with polygonal or fusiform form. It was found that inflammatory reactions were alleviated gradually in the early stage . There was an increasing angiogenesis at late stage. Materials degraded and absorbed more slowly. Conclusion The present study sug?gests that the novel 3-D composite bionic network has good histocompatibility with easy vascularization, and will be a candi?date scaffold for bone tissue engineering.
