Assessing Radiosensitivity of Lung Cancer with the Expression ofγH2AX
10.3969/j.issn.0253-9896.2014.09.005
- VernacularTitle:γH2AX对肺癌放射敏感性的预测效果
- Author:
Baolin HAN
;
Xuan WANG
;
Lifang LI
;
Yongzhi WANG
;
Guangshun WANG
- Publication Type:Journal Article
- Keywords:
radiation tolerance;
γH2AX protein;
lung cancer;
radiosensitivity
- From:
Tianjin Medical Journal
2014;(9):867-869
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the relationship between expression changes of γH2AX and the radiosensitivity of lung cancer cells in vitro. Methods The radiosensitivity of lung cancer cell lines A549 and SBC-3 was measured by clone forming assay. The DSBs damage of lung cancer cell lines A549 and SBC-3 was determined by Western blot assay. Re-sults The clone forming rates of lung cancer cell lines A549 and SBC-3 were gradually decreased with the increased radia-tion dose.γH2AX expression was related to the cell radiosensitivity 1 hour and 6 hours after radiated. Conclusion The phosphorylated histoneγH2AX is a powerful tool to monitor DNA DSBs and to predict the radiosensitivity in lung cancer ra-diotherapy.
