How to improve the pregnancy outcome by optimizing embryo cryopreservation protocol
10.3969/j.issn.0253-9896.2015.03.021
- VernacularTitle:如何优化胚胎冻存方案改善患者妊娠结局
- Author:
Yue ZHANG
;
Yunshan ZHANG
;
Fengxia XUE
- Publication Type:Journal Article
- Keywords:
frozen-thawed embryo transfer(FET);
vitrification;
slow-freezing;
optimal embryo;
cumulative pregnancy rate
- From:
Tianjin Medical Journal
2015;(3):300-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how to optimize the protocol of embryo cryopreservation to improve the success of frozen-thawed embryo transfer (FET), reduce multiple pregnancy rate and increase the cumulative pregnancy rate from one oocyte retrieval process. Methods The clinical data of 1 166 FET cycles were retrospectively analyzed and separated into different groups:445 for vitrification and 721 for slow-freezing. The vitrification group was divided into single embryo (28 cy?cles), double embryos (71 cycles) and triple embryos (346 cycles). 0-1 optimal embryo was called O0-1 group (235 cycles), 2 optimal embryos were called O2 group (80 cycles), 3 optimal embryos were called O3 group (130 cycles). The difference preg?nancy outcomes (implantation rate, clinical pregnancy, abortion rate and live-birth rate) were compared between groups. Results (1) There were significantly higher embryo survival rate(98.3%vs 73.1%), embryo recovery rate without damaging (83.3%vs 62.1%), implantation rate(36.8%vs 29.9%), clinical pregnancy(57.1%vs 44.0%) and live-birth rate(47.9%vs 34.5%) in vitrification group than those of slow freezing group(P<0.05). (2) There were significantly higher clinical pregnan?cy rate(62.7%vs 39.4%vs 32.1%), multiple pregnancy rate(37.6%vs 10.7%vs 0%)and live-birth rate(52.6%vs 31.0%vs 21.4%)in triple embryos group than those of the single and double embryos groups (P<0.05). (3) There were significantly higher implantation rate(33.0%vs 27.1%), clinical pregnancy(65.2%vs 48.1%), multiple pregnancy rate(38.0%vs 20.4%) and live-birth rate(56.2%vs 39.2%) in O2-3 group than those of O0-1 group(P<0.05). (4) There were significantly higher im?plantation rate(36.8%vs 26.3%) and multiple pregnancy rate (46.7%vs 21.3%) in O3 group than those of O2 group (P<0.05). There were no significant differences in clinical pregnancy(69.2%vs 58.8%), abortion rate(15.6%vs 10.6%), ectopic preg?nancy rate(0 vs 0), live-birth rate(58.5%vs 52.5%) and preterm birth rate(26.3%vs 14.3%) between two groups (P>0.05). Conclusion Vitrification technology can improve the clinical pregnancy and live-birth rate, and decrease multiple preg?nancy rate. Two optimal embryos in one tube are supposed to be the preferred method for embryo cryopreservation.
