Study of the Effects of Improved Wright-Giemsa Staining

VernacularTitle:改良瑞-姬氏染色法的染色效果研究
Author: Xingya SHEN ; Haowei ZHANG ; Guangming ZHAO ; Hexi WEI ; Lishun YANG
Publication Type:Journal Article
Keywords: spermatozoa; vaginal smears; trichomonas vaginalis; staining and labeling; improved wright-giemsa staining
From: Tianjin Medical Journal 2014;(9):931-932
CountryChina
Language:Chinese
Abstract: Objective To study the effects of improved Wright-Giemsa staining. Methods 40 semen samples by both traditional and improved Wright-Giemsa staining.The morphological characteristics stained by two methods was ob-served. Results The defective rates of the middle and tail parts of sperm were 7.02%± 2.4%and 11.02%± 2.03%respec-tively in improved staining group;while those were 5.48%±2.8%and 8.05%±2.56%in traditional staining group. There was statistical significance between two staining methods (P < 0.05). There was no significant difference of normal sperm and sperm head between these two staining methods;however, improved staining method showed a much clearer nucleus and ac-rosome staining than that of traditional methods. Improved Wright-Giemsa staining is better for sperm, vaginal discharge and Trichomonas vaginalis. More importantly, axostyle and flagella were clearly stained with the improved method. Conclusion Improved Wright-Giemsa staining is simple and can be used in clinical diagnosis.