Role of FAT/CD36 in high-fat diet-induced adipose tissue inflammation
10.3969/j.issn.1000-4718.2015.03.014
- VernacularTitle:FAT／CD36在高脂喂养小鼠脂肪组织炎症中的作用
- Author:
Yanyan ZHANG
;
Lei ZHAO
;
Yunxia XIE
;
Yaxi CHEN
;
Xiongzhong RUAN
- Publication Type:Journal Article
- Keywords:
Fatty acid translocase/CD36;
High-fat diet;
Adipose tissues;
inflammation
- From:
Chinese Journal of Pathophysiology
2015;(3):463-467
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of fatty acid translocase/CD36 (FAT/CD36) in adipose tissue in-flammation induced by a high-fat diet.METHODS:C57BL/6J mice were fed with a normal-chow diet ( NCD) or a high-fat diet ( HFD) for 14 weeks.The content of free fatty acid ( FFA) in the serum was measured by ELISA.The expression of CD36, cytokines and chemokines at mRNA and protein levels in the adipose tissues was determined by real-time poly-merase chain reaction and Western blotting.Immunohistochemical staining was used to examine the macrophages infiltration in the adipose tissues.The inflammatory responses in CD36 knockout mice and wild type mice with high-fat diet were ana-lyzed.RESULTS:The levels of FAT/CD36 were higher in HFD group than that in NCD group.HFD feeding enhanced the mRNA and protein expression of IL-1β, IL-6, TNF-α, MCP-1 and MIP-1, as well as promoted macrophage infiltration in the adipose tissues.Interestingly, as fed with HFD, the expression of cytokines/chemokines and macrophage infiltration were significantly reduced in adipose tissues of the CD36 knockout mice, compared with the wild type mice.CONCLU-SION:High-fat diet promotes adipose tissue inflammation in the mice in a FAT/CD36-dependent manner.
