Disruption of circadian rhythms inhibits wound healing of corneal epithe-lium in mice

VernacularTitle:扰乱昼夜节律对小鼠角膜创伤修复的影响
Author: Peng LIU ; Jun LIU ; Yunxia XUE ; Fang SONG ; Hanqing WANG ; Ting FU ; Chaoyong XIA ; Zhijie LI
Publication Type:Journal Article
Keywords: Cornea; Circadian rhythms; Wound healing; Inflammation
From: Chinese Journal of Pathophysiology 2015;(3):499-504
CountryChina
Language:Chinese
Abstract: [ ABSTRACT] AIM: To observe the effect of circadian rhythms on wound healing of mouse corneal epithelium. METHODS:The C57BL/6 male mice were used in the study.A part of corneal epithelium (2 mm in diameter) was struck off by a golf-like knife to form a round wound area.The dynamics of epithelial healing in the wound area were ob-served under microscope with fluorescein staining.In addition, with related antibodies and DAPI, the dynamic changes of the neutrophils, platelets and dividing cells were also investigated.RESULTS:The healing rates in LL group (12 h light/12 h light) and DD group (12 h dark/12 h dark) were obviously slower than that in LD group (12 h light/12 h dark), mainly showing delayed re-epithelialization, decreased epithelial cells, increased diameter of blood vessel, and delayed re-cruitment of neutrophils and platelets, but more cell number.CONCLUSION:Disruption of circadian rhythms significant-ly inhibits the wound healing of corneal epithelium, mainly through delaying the inflammation and re-epithelialization, but aggravating the inflammatory responses.