Prognostic Indicators for Infants Infected HCMV Virus after Ganciclovir Treatment
10.3969/j.issn.0253-9896.2014.09.026
- VernacularTitle:更昔洛韦治疗婴儿人巨细胞病毒感染的预后评估指标的探讨
- Author:
Juan LI
- Publication Type:Journal Article
- Keywords:
cytomegalovirus;
infant;
Ganciclovir;
IE mRNA;
pp67 mRNA;
virus identification
- From:
Tianjin Medical Journal
2014;(9):940-942
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the IE and pp67 mRNA expressions in infants infected human cytomegalovirus (HCMV) after ganciclovir treatment. Methods The values of total bilirubin (TBIL), direct bilirubin (DBIL), alanine amino-transferase (ALT) and alkaline phosphatase (ALP) were detected in patients with HCMV before treatment, 2 weeks and 4 weeks after treatment. The HCMV-DNA was detected by quantitative PCR. The expression levels of IE mRNA and pp 67 mRNA were detected by real-time fluorescent quantitative PCR. Results HCMV DNA stopped copying and the infected in-fants were cured following the traditional criterion, however, RNA expression was still tested in part of these infants. Conclusion It should be identified for mRNA expression when HCMV-DNA copies were hardly identified in these infants to prevent from relapse.
