Diagnostic Value of Combined Detection of the Level of IFN-γand IP-10 by Liquid Array Technology in Tuberculous Pleural Effusion
10.3969/j.issn.0253-9896.2014.09.027
- VernacularTitle:液态芯片技术联合检测IFN-γ和IP-10诊断结核性胸腔积液
- Author:
Jiaqing LIU
;
Li ZHANG
;
Haibai SUN
;
Min WU
;
Yanqing DU
;
Shuang FENG
;
Shuye LIU
- Publication Type:Journal Article
- Keywords:
interferon type Ⅱ;
chemokine CXCL10;
pleural effusion,malignant;
tuberculosis;
ROC Curve;
T-SPOT.TB;
liquid array technology;
IFN-γ-inducible protein 10
- From:
Tianjin Medical Journal
2014;(9):943-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the diagnostic value of combined detection of the liquid array technology, interfer-on (IFN)-γand IFN-γ-inducible protein (IP)-10 in the rapid, accurate diagnosis and differential diagnosis of tuberculous pleural effusions. Methods Patients with transudative pleural effusions were divided into tuberculous pleural effusion group (n=52) and malignant pleural effusion group (n=38). The method of T-SPOT.TB was used to detect the number of effec-tor T cells sensitized to Mycobacterium tuberculosis and spot forming cells (SFCs). The liquid array technology was used to detect the level of IFN-γand IP-10. Logistic regression was used to analyze and compare the diagnostic value of the two-method combination. Results The diagnostic sensitivity, specificity and the area under the ROC curve (AUC) of T-SPOT. TB were 90.38%, 84.21%, and 0.938 (95%CI:0.867-0.978), respectively. The diagnostic sensitivity, specificity and AUC of combined detection of IFN-γand IP-10 were 98.08%, 97.37%, and 0.995 (95%CI:0.951-1.000), respectively. There was no significant difference in the diagnostic sensitivity and specificity between the two methods, and the diagnostic agreement for the two diagnostic methods was fine (Kappa=0.703). The difference of AUC between the methods was significantly differ-ent (Z=1.996, P<0.05). The method of combined detection of IFN-γand IP-10 showed the larger AUC (AUC=0.995). Con-clusion The combined diagnosis meets the clinical needs of rapid, accurate diagnosis and differential diagnosis for tuber-culous pleural effusion by simultaneously assaying the level of IFN-γand IP-10 using the liquid array technology.
