Cloning and synthesis of a new A-superfamily conotoxin Bt14.10
10.7644/j.issn.1674-9960.2014.08.008
- VernacularTitle:新A超家族芋螺毒素Bt14．10的克隆及合成研究
- Author:
Fei WANG
;
Longxiao ZHANG
;
Liang LI
;
Zhuguo LIU
;
Qiuyun DAI
- Publication Type:Journal Article
- Keywords:
A-superfamily conotoxin;
cloning;
intron;
synthesis;
disulfide-bridges
- From:
Military Medical Sciences
2014;(8):598-601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone a new conotoxin Bt14.10 from Conus betulinus derived from the South China Sea, synthesize the peptide , and to determine linkage of its disulfide bridges .Methods The genomic DNA was extracted from C.betulinus venom duct while the Bt14.10 sequence was cloned using primers designed based on the untranslated region and intron.The peptide was then synthesized using solid-phase method and folded into the target product whose disulfide bridge connection was further determined by two-step oxidative folding .Results A novel conotoxin designated as Bt 14.10 (CAHSVPGMHPCKCNNTC-NH2) was obtained,the disulfide connectivity of which was C1-C3,C2-C4.Conclusion Bt14.10 is a new A-superfamily conotoxin and has a distinct loop spacing pattern between cysteines in A-superfamily conotoxins.
