Protein-RNA interactions in Escherichia coli:a genome-wide study

VernacularTitle:大肠杆菌基因组水平蛋白质-RNA相互作用初步研究
Author: Song XU ; Yaowen CHEN ; Xiaomin YING ; Hanjiang FU ; Baolei TIAN ; Yi SONG ; Xiaofei ZHENG ; Wuju LI
Publication Type:Journal Article
Keywords: protein-RNA interactions; sRNA; E.coli; MCA
From: Military Medical Sciences 2014;(8):612-616
CountryChina
Language:Chinese
Abstract: Objective To conduct a pilot study on genome-wide in vivo protein-RNA interactions in E.coli.Methods Bacterial lysate was treated with RNase before the RNA fragments protected by proteins were extracted from treated lysate and used to construct cDNA library that was applied to high-throughput sequencing .Finally, the transcripts bound by proteins were obtained by bioinformatics analysis .Results A total of 3193 transcripts were obtained , including 2234 mRNAs, 47 sRNAs, 39 tRNAs, 11 rRNAs, and 862 intergenic regions .Conclusion Some information of transcripts interacting with proteins in E.coli is acquired , which will facilitate further studies of protein-RNA interactions .