Proteomics study on liver of acute hepatic failure rats
10.3969/j.issn.1671-8348.2015.12.003
- VernacularTitle:急性肝衰竭大鼠肝脏的蛋白质组学研究
- Author:
Lei CAI
;
Wailin HOU
;
Yuan CHENG
;
Mingxin PAN
;
Yi GAO
- Publication Type:Journal Article
- Keywords:
rats;
liver failure;
acute;
proteomics;
electrophoresis;
gel;
two-dimensional;
spectrometry;
mass;
matrix-assisted laser desorption-ionization
- From:
Chongqing Medicine
2015;44(12):1592-1595
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the differentially expressed proteinin the livetissue of the drug-induced acute hepatifail-ure rat.MethodTwenty-foumale SD ratwere randomly divided into two group,the experimental group (12 cases) wagiven D-galactosamine10g/L by intraperitoneal injection and the control group (12 cases) wagiven normal saline by intraperitoneal injec-tion .The total proteinin the livetissue samplewere extracted ,quantitated ,and subjected to separate by the two-dimension elec-trophoresis(2-DE) of isoelectrifocusing (IEF) and SDS-PAGE ,found outhe discrepanprotein spotby the software and per-formed the identification by MALDI-TOF-M.Result27 differential protein spotwere successfully identified ,and 15 up-regula-ted and 12 down-regulated proteinexpressionwere obtained in the experimental group compared with the control group .Conclu-sion The significandifferencein the expressionof protein,such acasein kinase I(CKⅠα) ,tyrosine protein kinase(PTK) ,pro-liferating cell nucleaantigen(PCNA) ,et.in the liveexisbetween the acute hepatifailure model ratand the normal one.
