Role of GSK-3βin podocyte transdifferentiation effect under high glucose condition
10.3969/j.issn.1671-8348.2015.12.008
- VernacularTitle:GSK-3β在高糖环境下足细胞转分化效应中的作用
- Author:
Lili YANG
;
Zhangsuo LIU
- Publication Type:Journal Article
- Keywords:
podocytes;
glycogen synthase kinase 3;
cell transdifferentiation;
transfection .
- From:
Chongqing Medicine
2015;44(12):1609-1612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the action of GSK-3βin podocyte transdifferentiation effecundethe high glucose condition . MethodPodocytewere treated in RPMI-1640 medium with differenconcentrationof glucose fo36 h .The expressionof neph-rin ,podocin ,α-Smand Fibronectin were detected by both Western bloand indirecimmunofluorescence analysi.Athe same time ,the change of albumin inflow amounof podocytein differentreatmengroupwadetected by using the Transwell cham-be.The changeof expression amounand activity of GSK-3βin high glucose condition were detected by using the GSK-3 activity assay ki.The phenotypiand functional changeof podocytewere detected aftedisturbing GSK-3βby GSK-3βsiRNin the high glucose group .ResultThe expression levelof nephrin and podocin protein were down-regulated with the increase of the glucose concentration in dose-dependenmanner(P＜0 .05) ,and the expression level of α-Smwaup-regulated with the increase of the glucose concentration in dose-dependenmanner(P＜0 .05);the albumin inflow amounof podocytewaup-regulated with the in-crease of the glucose concentration in dose-dependenmanner(P＜0 .05) .The expression amounof GSK-3βin the high glucose group waincreased(P＜0 .05) .Compared with the control group ,the expression amounof nephrin and podocin aftehigh glucose treatmenin the GSK-3β siRNtreatmengroup waincreased ,while the expression of α-Smwadecreased .Conclusion The high glucose condition could induce the phenotypichange and functional impairmenof mice podocyte;GSK-3βparticipatein the epithelial-mesenchymal transdifferentiation procesof podocyte undethe high glucose condition .
