Proteomic analysis of energy metabolism-related proteins of mice midbrain
10.3969/j.issn.1673-4130.2015.13.019
- VernacularTitle:蛋白质组学技术分析小鼠中脑能量代谢蛋白
- Author:
Guohua DU
;
Hongxu WANG
;
Ziliang LIU
- Publication Type:Journal Article
- Keywords:
midbrain;
energy metabolism-related proteins;
proteomics;
two-dimensional gel electrophoresis;
matrix-as-sisted laser desorption/ionization time-of-flight mass spectrometry
- From:
International Journal of Laboratory Medicine
2015;(13):1846-1848
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify energy metabolism-proteins of mice midbrain by using the proteomic technique,and to investi-gate the relationship between these proteins and neural diseases.Methods Two-dimensional gel electrophoresis was used to sepa-rate totally soluble proteins extracted from mice midbrain.Some protein spots on two-dimensional gel electrophoresis gels were ana-lyzed by using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).Results 24 protein spots related with energy metabolism were separated by two-dimensional gel electrophoresis and identified by using MALDI-TOF MS successfully.Conclusion The establishment of energy metabolism-related proteins map of mice midbrain lays a foundation for the research on the involvement of these proteins in neural disease pathogenesis.
