Analysis of factors affecting results of ALT preliminary screening in blood donors
10.3969/j.issn.1673-4130.2015.13.030
- VernacularTitle:影响献血者 ALT 初筛结果的原因分析
- Author:
Yan GAO
;
Huojiao CHE
;
Yongping MAI
;
Jianyun WU
- Publication Type:Journal Article
- Keywords:
dry chemical method;
alanine aminotransferase;
preliminary screening;
quality control;
blood donors
- From:
International Journal of Laboratory Medicine
2015;(13):1872-1873
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the factors affecting results of alanine aminotransferase(ALT)preliminary screening by using dry chemical method in blood donors,so as to establish standards of ALT preliminary screening and reduce waste of blood. Methods A total of 21 065 blood specimen of blood donors in Blood Center of Maoming City were collected from January to June 2013.The influences of specimen hemolysis,lipidemia,different sample amount and waiting time after adding sample on the detec-tion results of ALT by using dry chemical method were comparatively analysed,and standardized procedures and established appro-priate limits of ALT preliminary screening according to the results.Results The specimen hemolysis affected test results of ALT, which was not obvious when the hemolysis rate was less than or equal to 0.5%.There was no obvious interference of lipidemia on ALT test.When the sample amount was (32±3)μL or the waiting time after adding sample was 10 to 40 seconds,the detection re-sults of ALT were acceptable.The standard limit of ALT was less than or equal to 40 U/L of female and less than or equal to 45 U/L of male.Conclusion Hemolysis,sample amount and waiting time after adding sample can influence the results of ALT detec-ted by using dry chemical method,while lipidemia had no interference on ALT test.Standardizing operation and reasonably setting normal limits standard may ensure the accuracy and stability of determination,save blood resources,further ensure blood safety, which should be worthy of wide application.
