Research on Optimization of Extraction Process of Formula Granules of Clematis chinensis Osbeck by Orthogonal Design and Its Qualitative Discrimination

VernacularTitle:正交试验法优化威灵仙配方颗粒提取工艺及定性鉴别研究
Author: Ying ZHOU ; Ping ZHOU
Publication Type:Journal Article
Keywords: formula granules of Clematis chinensis Osbeck; orthogonal design; extraction process; HPLC; TLC
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(10):2274-2277
CountryChina
Language:Chinese
Abstract: This study was aimed to optimize extraction process of formula granules of Clematis chinensis Osbeck and establish its qualitative discrimination method. With oleanolic acid, hederagenin and extract rate as indexes, extraction technology conditions of formula granules of C. chinensis Osbeck were investigated by L9 (34) orthogonal test method. TLC was used in the qualitative discrimination of formula granules of C. chinensis Osbeck. The results showed that optimum extraction technology was: adding 12 times of water; soaking for 0.5 h; cooking for 1 h; and extracting for 3 times. TLC had the diagnostic characteristic for the distinct spot which illustrated the specificity. It was concluded that the extraction process was reasonable, simple and feasible with high extraction efficiency of ef-fective contents, which can be the scientific basis for production and quality control of formula granules of C. chin-ensis Osbeck.