Determination of Scopoletin in Herba Artemisiae hedinii by HPLC
10.11842/wst.2014.10.031
- VernacularTitle:HPLC法测定臭蒿中东莨菪内酯的含量
- Author:
Xiaozhen LIU
;
Zhiping TONG
;
Rui TAN
;
Jun WAN
;
Song XIE
- Publication Type:Journal Article
- Keywords:
Artemisiae hedinii;
scopoletin;
HPLC;
chromatography
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(10):2233-2235
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, a qualitative and quantitative analysis method was established for the scopoletin in herba Artemisiae hedinii hy HPLC. The chromatographic column was Agilent TC-C18 (4.6 mm×250 mm, 5μm);the tem-perature of column was 30℃, eluted with a mobile phase consisted of methanol and 0.3%phosphoric acid solution and gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was 344 nm. The results showed that the mass concentration and peak area of scopoletin had a good linear relationship within the range of measurement (r=0.999 9). The reproducibility was good;and the RSD was 1.51%. The average sample recovery rate was 101.42%. It was concluded that the established method was stable, reliable and simple. It provided theoretical evidences for the quality evaluation and quality control of herba A. hedinii.
