Expression Profile and Bioinformatics Studies of Long Noncoding RNA in Mouse Embryonic Fibroblasts under Endoplasmic Reticulum
10.11969/j.issn.1673-548X.2015.04.017
- VernacularTitle:小鼠胚胎成纤维细胞内质网应激反应中的长链非编码RNA表达谱及生物信息学研究
- Author:
Hongyang QUAN
;
Qianqian FAN
;
Lin WANG
- Publication Type:Journal Article
- Keywords:
Long noncoding RNA;
Endoplasmic reticulum stress response;
Microarray;
Bioinformatics
- From:
Journal of Medical Research
2015;44(4):61-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential significance of long noncoding RNA (lncRNA) expressed in endoplasmic reticulum stress response also known as unfolded protein response ( UPR) .Methods Mouse embryonic fibroblasts ( MEFs) were isolated from 13 day mouse embryo , cultured and treated with tunicamycin for 16 hours to induce UPR .The untreated MEFs were used as negative control .The lncRNA expression profile was examined by a customized lncRNA array. The results of array assay were validated by real-time PCR and further analyzed by bioinformatics. Results There were 411 lncRNAs whose expression was signficantly up-regulated in MEFs treated with tunicamycin as compared with the controls ,while 790 lncRNAs were significantly down-regulated. A number of significantly alter lncRNAs were validated by real-time PCR. A further bioinformatics analysis of lncRNA profile suggested that many cold be in-volved in multiple cellular pathways. Conclusion Our lncRNA expression profile and bioinformatics studies srongly suggest that many lncRNAs can be regulated in the cells under UPR and could also in turn regulate UPR and other cellular processes.
