Apelin-13 protects the cerebral ischemia-reperfusion injury in rats
10.11958/j.issn.0253-9896.2015.05.010
- VernacularTitle:Apelin-13对大鼠脑缺血-再灌注损伤的保护作用
- Author:
Xin DONG
;
Suqing LU
;
Huiying LIAO
;
Xinping OUYANG
;
Guoshu LI
;
Jie ZHOU
- Publication Type:Journal Article
- Keywords:
Apelin-13;
cerebral ischemia-reperfusion injury;
nerve function;
cerebral infarction;
endoplasmic reticulum stress;
glucose-regulated protein 78;
CCAAT/enhancerbinding protein homologous protein
- From:
Tianjin Medical Journal
2015;43(5):484-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of Apelin-13 on the cerebral ischemia-reperfusion injury (CIRI), and to explore the possible mechanism in rat model. Methods Fifty male SD rats were randomly divided into five groups:sham group, CIRI model group and Apelin-13 (0.1, 1.0 and 10.0 μg/kg) treatment groups. The model of CIRI was established by filament. After 2 h ischemia, the focal middle cerebral artery was followed by 72 h reperfusion. Apelin-13 was administrated by intracerebroventricular injection 30 minutes before reperfusion. The score of neural function was estimated in different time points. The 2,3,5-triphenyl tetrazolium chloride (TTC) dye was used to calculate the volume and percentage of cerebral infarction. The endoplasmic reticulum stress (ERS) protein markers including glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP) in cerebral cortex were measured by Western blot assay. Results Compared with the sham group, the score of neural function was significantly increased, the infarct rate was reached(47.63 ± 5.81)%and the protein expressions of GRP78 and CHOP were significantly up-regulated in CIRI model group (P＜0.05). There were no significant differences in these data between the CIRI model group and 0.1 μg/kg Apelin-13 treatment group (P＞0.05). Compared with the CIRI group, the neural function defect was significantly improved, the muscle strength was significantly enhanced and the infarct rate was significantly decreased, and the protein expressions of GRP78 and CHOP were significantly down-regulated in the 1.0 and 10.0 μg/kg Apelin-13 treatment groups (P＜0.05). Conclusion Apelin-13 protects the cerebral ischemia-reperfusion injury in rat model, which may be related with the inhibition of endoplasmic reticulum stress.
