Isolation and Culture of Epidermal Stem Cells from the Third Generation of Mice That Were Knocked-out of Terc
10.3969/j.issn.0253-9896.2014.08.008
- VernacularTitle:第三代端粒酶缺失小鼠皮肤表皮干细胞分离培养特性观察
- Author:
Junling ZHANG
;
Song HUANG
;
Lu LU
;
Deguan LI
;
Hongying WU
;
Aimin MENG
- Publication Type:Journal Article
- Keywords:
mice,knockout;
skin aging;
stem cells;
telomerase
- From:
Tianjin Medical Journal
2014;(8):762-764
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the difference in number of epidermal stem cell and its function between wild-type (WT) mice and the third generation of Terc knockout (G3Terc-/-) mice. Methods Flowcytometry was used to analyse and sort epidermal stem cells;Quantitative real-time PCR is used to analyse the relative expression level of p 21 in epider-mal stem cells;Self-renewal ability was reflected by the number of colonies formed by epidermal stem cells. Results Basal and suprabasal ratios in epidermal stem cells in WT mice were (9.56 ± 1.06)% and (1.22 ± 0.08)% respectively; basal and suprabasal ratios in epidermal stem cell in G3Terc-/-mice were (17.36±3.56)%and (2.92±0.72)%respectively. Relative p21 expression level in G3Terc-/-mice was 6.40 fold to WT mice;Number of colonies formed by WT mice epidermal stem cells were (280.20±29.81) per 104 cell, number of colonies formed by G3Terc-/-mice epidermal stem cells were (29.28±5.24) per 104 cell, which present significant difference to each other(P<0.05). Conclusion Compared to WT mice, epidermal stem cells in G3Terc-/-mice were aging.
