The Protective Effect of Ebselen on Mitochondrial Damage after Spinal Cord Injury in Rats
10.3969/j.issn.0253-9896.2014.08.009
- VernacularTitle:依布硒啉对大鼠急性脊髓损伤后线粒体的保护作用
- Author:
Zhiqiang JIA
;
Yansong WANG
;
Gang LI
;
Zhongkai FAN
;
Chenchong ZHANG
;
Shuai HE
;
Gang LYU
- Publication Type:Journal Article
- Keywords:
spinal cord injuries;
mitochondria;
malondialdehyde;
glutathione;
cytochromes c;
neurons;
apoptosis;
eb-selen
- From:
Tianjin Medical Journal
2014;(8):765-768
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Ebselen on mitochondrial damage and its influence to Cytochrome C expression and the neuronal apoptosis after spinal cord injury in rats. Methods Sixty adult SD rats were ran-domly divided into 5 groups (12 each group). Spinal cord injury model was made using Allen's method. Sham operation group received only laminectomy;SCI group received laminectomy and spinal trauma;Saline group received saline injection intraperitoneally (0.1%DMSO) after injury;methylprednisolone group received 30 mg/kg methylprednisolone injection intra-peritoneally, ebselen group received 10 mg/kg ebselen injection intraperitoneally. The malonaldehyde (MDA) and glutathi-one (GSH)level at the injured sites of the spinal cord were detected 24 hours after trauma, and the expression level of Cyto-chrome C was also observed. Finally, neuronal apoptosis was identified by TUNEL staining. Results MDA level in the Eb-selen group was significantly lower than that in the SCI group, and GSH level was significantly elevated in the Ebselen group compared with SCI group (P<0.01). Expression of Cytochrome C in Ebselen group was lower than that in SCI group shown by Western blot, and the neuronal apoptosis in Ebselen group reduced significantly too compared with SCI group (P<0.01). Conclusion Ebselen can alleviate peroxidation,prohibit expression of Cytochrome C and inhibit neuronal apoptosis,thus it shows a protective effect to experimental acute SCI.
